Radiation damage in protein crystallography: susceptibility study

Thesis

Protein structure models obtained from X-ray crystallography are subject to radiation damage. The resulting specific alterations to protein structures can be mistaken for biological features, or may obscure actual protein mechanisms, leading to misidentification or obscuration of biological insight. The radiation chemistry behind this site-specific damage is not well understood.

Radiation damage processes progress in proportion to the dose absorbed by the crystal in the diffraction ...

Expand abstract

Authors: Gerstel, M

• Publication date: 2014
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• Language: English
• Keywords: radiation damage; specific damage; preferential damage; atomic displacement parameters; atomic B factors
• Subjects: Structural chemistry; Crystallography; Protein chemistry; Bioinformatics (biochemistry); Biochemistry; Radiation chemistry