Investigating DNA replication perturbance induced by CRISPR-Cas9 variants

Thesis

Single-Stranded DNA breaks (SSBs) represent one of the most abundant DNA lesions occurring in the cells of living organisms. If encountered by the DNA replication machinery, SSBs can be converted into double-stranded DNA breaks (DSBs). Homologous recombination (HR) pathways are deployed to repair these DSBs and initiate replication restart. However, HR-mediated replication restart, whilst helping to ensure that DNA replication is completed prior to cell division, is a potential cause of genom...

Expand abstract

Authors: Xu, Y

• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• DOI: 10.5287/ora-b7zx5bj0x
• Language: English
• Keywords: Replication perturbance at Cas9/Cas9n induced SSBs/DSBs; Cas9d; Replication perturbance at catalytically dead Cas9; as a R-loop associated DNA-binding protein barrier; CRISPR-Cas9
• Subjects: Molecular genetics; Biochemistry