Thesis
Advanced data analysis of single-molecule fluorescence signals: from viral particles to DNA sequencing
- Abstract:
-
Single-molecule fluorescence microscopy is a powerful tool in the life sciences, enabled by advances in detectors, fluorophore and sequence design, and analysis methods. It reveals dynamic processes, intermediates, and molecular subpopulations that are invisible to ensemble measurements. By analysing the diffraction-limited image of an isolated fluorophore, emitter positions can be determined with high precision, forming the basis of SMLM (single-molecule localisation microscopy). SMLM yields...
Expand abstract
Actions
Access Document
- Files:
-
-
(Preview, Dissemination version, pdf, 96.5MB, Terms of use)
-
Authors
Contributors
+ Hazra, J
- Institution:
- University of Oxford
- Division:
- MPLS
- Department:
- Physics
- Sub department:
- Condensed Matter Physics
- Role:
- Contributor
+ Kümmerlin, M
- Institution:
- University of Oxford
- Division:
- MPLS
- Department:
- Physics
- Sub department:
- Condensed Matter Physics
- Role:
- Contributor
+ Hepp, C
- Institution:
- University of Oxford
- Division:
- MPLS
- Department:
- Physics
- Sub department:
- Condensed Matter Physics
- Role:
- Contributor
+ Kapanidis, A
- Institution:
- University of Oxford
- Division:
- MPLS
- Department:
- Physics
- Sub department:
- Condensed Matter Physics
- Role:
- Supervisor
- ORCID:
- 0000-0002-0904-5323
+ Berry, R
- Institution:
- University of Oxford
- Division:
- MPLS
- Department:
- Physics
- Sub department:
- Condensed Matter Physics
- Role:
- Examiner
- ORCID:
- 0000-0002-0331-473X
- DOI:
- Type of award:
- DPhil
- Level of award:
- Doctoral
- Awarding institution:
- University of Oxford
- Language:
-
English
- Keywords:
- Subjects:
- Deposit date:
-
2026-04-23
- ARK identifier:
Terms of use
- Copyright holder:
- Qing Zhao
- Copyright date:
- 2025
- Notes:
- High-throughput single-virion DNA-PAINT reveals structural diversity, cooperativity, and flexibility during selective packaging in influenza, Tunable fluorogenic DNA probes drive fast and high-resolution single-molecule fluorescence imaging, and Unraveling single-molecule reactions via multiplexed in-situ DNA sequencing are derived from this thesis.
If you are the owner of this record, you can report an update to it here: Report update to this record