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A failure of meiotic chromosome segregation in a fbh1Delta mutant correlates with persistent Rad51-DNA associations.

Abstract:
The F-box DNA helicase Fbh1 constrains homologous recombination in vegetative cells, most likely through an ability to displace the Rad51 recombinase from DNA. Here, we provide the first evidence that Fbh1 also serves a vital meiotic role in fission yeast to promote normal chromosome segregation. In the absence of Fbh1, chromosomes remain entangled or segregate unevenly during meiosis, and genetic and cytological data suggest that this results in part from a failure to efficiently dismantle Rad51 nucleofilaments that form during meiotic double-strand break repair.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/nar/gkq977

Authors

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Institution:
University of Oxford
Division:
MSD
Department:
Biochemistry
Role:
Author


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Funding agency for:
Whitby, M
Grant:
057586/Z/99/B


Publisher:
Oxford University Press
Journal:
Nucleic acids research More from this journal
Volume:
39
Issue:
5
Pages:
1718-1731
Publication date:
2011-03-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048


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