Journal article
Immunization with matrix-, nucleoprotein and neuraminidase protects against H3N2 influenza challenge in pH1N1 pre-exposed pigs
- Abstract:
- There is an urgent need for influenza vaccines providing broader protection that may decrease the need for annual immunization of the human population. We investigated the efficacy of heterologous prime boost immunization with chimpanzee adenovirus (ChAdOx2) and modified vaccinia Ankara (MVA) vectored vaccines, expressing conserved influenza virus nucleoprotein (NP), matrix protein 1 (M1) and neuraminidase (NA) in H1N1pdm09 pre-exposed pigs. We compared the efficacy of intra-nasal, aerosol and intra-muscular vaccine delivery against H3N2 influenza challenge. Aerosol prime boost immunization induced strong local lung T cell and antibody responses and abrogated viral shedding and lung pathology following H3N2 challenge. In contrast, intramuscular immunization induced powerful systemic responses and weak local lung responses but also abolished lung pathology and reduced viral shedding. These results provide valuable insights into the development of a broadly protective influenza vaccine in a highly relevant large animal model and will inform future vaccine and clinical trial design.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Version of record, pdf, 2.8MB, Terms of use)
-
(Preview, Supplementary materials, pdf, 314.1KB, Terms of use)
-
- Publisher copy:
- 10.1038/s41541-023-00620-2
Authors
+ The Pirbright Institute
More from this funder
- Funder identifier:
- https://ror.org/04xv01a59
- Grant:
- BBS/E/I/00007039
- BBS/E/I/00007037
- BBS/E/I/00007031
+ Medical Research Council
More from this funder
- Funder identifier:
- https://ror.org/03x94j517
- Grant:
- MR/S037160/1
- Publisher:
- Springer Nature
- Journal:
- npj Vaccines More from this journal
- Volume:
- 8
- Issue:
- 1
- Article number:
- 19
- Place of publication:
- England
- Publication date:
- 2023-02-15
- Acceptance date:
- 2023-01-30
- DOI:
- EISSN:
-
2059-0105
- Pmid:
-
36792640
- Language:
-
English
- Pubs id:
-
1328768
- Local pid:
-
pubs:1328768
- Source identifiers:
-
W4321002149
- Deposit date:
-
2026-04-01
- ARK identifier:
Terms of use
- Copyright holder:
- Vatzia et al.
- Copyright date:
- 2023
- Rights statement:
- © The Author(s) 2023. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
- Licence:
- CC Attribution (CC BY)
If you are the owner of this record, you can report an update to it here: Report update to this record