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Studies of enzyme kinetics and aspects of enzyme structure in vivo using NMR and molecular genetics

Abstract:

A quantitative understanding of metabolic control depends on a knowledge of the enzymes involved. The extrapolation of studies in vitro to the intact cell is controversial because the intracellular environment is relatively poorly characterised, particularly with respect to the interactions between weakly-associated enzymes.

There is a clear need to study enzymes directly in the cell, yet there are few suitable techniques. Metabolites have been very successfully studied in...

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Institution:
University of Oxford
Department:
Life and Environmental Sciences Division
Publication date:
1992
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
University of Oxford
Barcode:
603841307
URN:
uuid:d8baa574-a5d4-45a2-95a2-c141fbf8d277
Local pid:
td:603841307

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