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A 'Build and Retrieve' methodology to simultaneously solve cryo-EM structures of membrane proteins

Abstract:

Single-particle cryo-electron microscopy (cryo-EM) has become a powerful technique in the field of structural biology. However, the inability to reliably produce pure, homogeneous membrane protein samples hampers the progress of their structural determination. Here, we develop a bottom-up iterative method, Build and Retrieve (BaR), that enables the identification and determination of cryo-EM structures of a variety of inner and outer membrane proteins, including membrane protein complexes of ...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41592-020-01021-2

Authors


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Role:
Author
ORCID:
0000-0001-5795-0232
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Institution:
University of Oxford
Department:
CHEMISTRY
Subgroup:
Physical & Theoretical Chem
Role:
Author
ORCID:
0000-0003-4346-182X
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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Subgroup:
Physical & Theoretical Chem
Oxford college:
Exeter College
Role:
Author
ORCID:
0000-0001-7829-5505
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Publisher:
Springer Nature Publisher's website
Journal:
Nature Methods Journal website
Volume:
18
Issue:
1
Pages:
69-75
Publication date:
2021-01-06
Acceptance date:
2020-11-16
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Pmid:
33408407
Pubs id:
1156867
Local pid:
pubs:1156867
Language:
English
Keywords:
Format:
Print-Electronic

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