NMR studies on 2-oxoglutarate oxygenases

Thesis

The work described in this Thesis has focused on the application of nuclear magnetic resonance (NMR) spectroscopy to study the Fe(II) and 2-oxoglutarate (2OG) dependent oxygenases, a family of structurally related enzymes that are ubiquitous in plants, micro-organisms and animals. The work included enzyme mechanistic studies, NMR method development, protein NMR and method development for inhibitor discovery.

NMR was applied to study human γ-butyrobetaine hydroxylase (BBOX), an enzym...

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Authors: Leung, IKH

• Publication date: 2013
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• Language: English
• Keywords: hypoxia; HIF; prolyl hydroxylase; nuclear magnetic resonance; protein-ligand interaction; PHD2; dynamic combinatorial chemistry; hypoxia inducible factor; oxygenase; enzyme inhibition; oxygen sensing; DCC; 2OG; NMR; 2-oxoglutarate; carnitine biosynthesis
• Subjects: Chemistry & allied sciences; Biochemistry; Biophysics; NMR spectroscopy; Biology; Enzymes; Chemical biology; Life Sciences; Protein chemistry; Biology (medical sciences); Biophysical chemistry; Catalysis