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Thesis

Probing protein structure and dynamics through native MS and H/D exchange

Abstract:

This thesis details the development and application of mass spectrometry (MS) labelling strategies for use in structural biology, and, specifically, how they offer insight into the quaternary structure and dynamics of protein complexes.

Chapter 3 first demonstrates the utility of isotopic H/D exchange (HDX) strategies for probing the in solution dynamics and assembly of two protein systems. Firstly, I use solution HDX to probe the light-induced structural change of the FAD-bind...

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Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Department:
Chemistry
Role:
Author

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Role:
Supervisor
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Funding agency for:
Chandler, S
Grant:
BB/L017067/1
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
University of Oxford
Subjects:
UUID:
uuid:cced3b96-638a-4dbb-bd34-5b66d335cbc3
Deposit date:
2019-09-16

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