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Thesis

Gas propulsion of microprojectiles for the transformation of biological cells

Abstract:

Bombardment of intact cells and tissue with DNA-coated microprojectiles represents a novel approach to the genetic transformation of biological material. In this thesis, a gas propulsion particle gun is developed for such a purpose. The particle gun utilises gas dynamics to control particle velocity and spread, thereby enabling optimisation of transformation efficiencies for varying types of target material. The dynamics associated with particle acceleration are shown to relate to transien...

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Peer review status:
Peer reviewed

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Department:
University of Oxford
Role:
Author
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
University of Oxford
UUID:
uuid:c78bb189-665d-4199-8a0b-ce0b439389c7
Local pid:
polonsky:1:39
Source identifiers:
603840975
Deposit date:
2017-10-04

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