Small-molecule fluorescence-based probes for interrogating major organ diseases

Journal article

With this research, we have developed two long-wavelength theranostic probes (DCMT and DCMC) with aggregation-induced emission (AIE)-based properties for image-guided photodynamic therapy (PDT) of hepatoma cells. Introduction of a triphenylamine or carbazole group to a dicyanomethylene-4H-pyran dye with long-wavelength fluorescence emission produces the AIE-based probes, which were subsequently modified with triphenyl-phosphonium cation for actively targeting the mitochondria of hepatoma cells. Solution-based experiments show that the probes exhibit a mixed photophysical mechanism of twisted-intramolecular charge transfer and AIE at different aggregation states. The molecular aggregation of the probes also leads to an enhanced ability for oxygen photosensitization, suggesting their potential for PDT of cancer cells. Our subsequent cell-based assays show that the probes localize in the mitochondria of hepatoma cells and the use of light leads to cell death through the intracellular production of reactive oxygen species.

Authors: Han, H-H; Tian, H; Zang, Y; Sedgwick, AC; Li, J

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Royal Society of Chemistry
• Journal: Chemical Society Reviews
• Volume: 50
• Issue: 17
• Pages: 9391-9429
• Publication date: 2021-09-07
• DOI: 10.1039/d0cs01183e
• EISSN: 1460-4744
• ISSN: 0306-0012
• Language: English
• Keywords: Computational biology; Nanotechnology; Optics; Chemistry; Materials science; Biology; Physics; Fluorescence