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Thesis

Engineered α-hemolysin pores with chemically and genetically-fused functional proteins

Abstract:

Protein engineering could be used to bring two proteins together, which don't normally interact, in an oriented configuration. Using computer modelling and experimental work involving mutagenesis, a new dimer complex, (α7)2, was engineered with two α-hemolysin (αHL) heptamers (α7) units linked via disulfide bridges in a cap-to-cap orientation. The structure of (α7)2 was confirmed by biochemical analysis, transmission electron microscopy (TEM) and single-channel electrical recording. Import...

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Institution:
University of Oxford
Oxford college:
St Cross College
Department:
Mathematical,Physical & Life Sciences Division - Chemistry - Chemical Biology
Role:
Author

Contributors

Role:
Supervisor
Publication date:
2013
Type of award:
DPhil
Level of award:
Doctoral
Awarding institution:
Oxford University, UK
URN:
uuid:ac99948c-9c3a-4388-a2fc-5955fd9446ee
Local pid:
ora:7145

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