Using NMR to study protein-ligand interactions

Thesis

The work described in this thesis focused on the use of nuclear magnetic resonance spectroscopy (NMR) to study two classes of metallo enzymes – the Fe(II)- and 2oxoglutarate (2OG)-dependent dioxygenases and the metallo β-lactamases (MBLs). These enzymes are involved in clinically important biological processes, i.e. the hypoxic response and antimicrobial resistance, respectively. Both protein systems are interesting from an NMR perspective because they have dynamic regions involve...

Expand abstract

Authors: Abboud, M

• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• Language: English
• Keywords: Lactams; Hypoxia; NMR; Prolyl hydroxylation; Antibiotic Resistance; Lactamases
• Subjects: Pharmaceutical chemistry; Nuclear magnetic resonance; Chemical Biology; Biochemistry