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Programmable polyproteams built using twin peptide superglues

Abstract:

Programmed connection of amino acids or nucleotides into chains introduced a revolution in control of biological function. Reacting proteins together is more complex because of the number of reactive groups and delicate stability. Here we achieved sequenceprogrammed irreversible connection of protein units, forming polyprotein teams by sequential amidation and transamidation. SpyTag peptide is engineered to spontaneously form an isopeptide bond with SpyCatcher protein. By engineering the adhe...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1073/pnas.1519214113

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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chemistry Laboratory
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Funding agency for:
Veggiani, G
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Funding agency for:
Veggiani, G
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Funding agency for:
Nakamura, T
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Publisher:
National Academy of Sciences Publisher's website
Journal:
Proceedings of the National Academy of Sciences of USA Journal website
Volume:
113
Issue:
5
Pages:
1202–1207
Publication date:
2015
DOI:
ISSN:
1091-6490
URN:
uuid:91a8ede3-f745-424a-9e14-4f14af2491cc
Source identifiers:
580878
Local pid:
pubs:580878

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