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Multicopy plasmids potentiate the evolution of antibiotic resistance in bacteria

Abstract:

Plasmids are thought to play a key role in bacterial evolution by acting as vehicles for horizontal gene transfer, but the role of plasmids as catalysts of gene evolution remains unexplored. We challenged populations of E. coli carrying blaTEM-1 β- lactamase gene on either the chromosome or a multicopy plasmid (19/cell) with increasing concentrations of ceftazidime. The plasmid accelerated resistance evolution by increasing the rate of appearance of novel TEM-1 mutations conferring resistance...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41559-016-0010

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Zoology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Zoology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Zoology
Role:
Author
More from this funder
Funding agency for:
San Millan, A
Grant:
Miguel Servet Fellowship MS15/00012
More from this funder
Grant:
FP7/2007-2013) StG-2011-645 281591
Publisher:
Springer Nature Publisher's website
Journal:
Nature Ecology and Evolution Journal website
Volume:
1
Pages:
0010
Publication date:
2016-11-07
Acceptance date:
2016-08-31
DOI:
EISSN:
2397-334X
Pubs id:
pubs:641887
UUID:
uuid:68a649fb-b18b-4a72-b24e-c26f1ff5bfa1
Local pid:
pubs:641887
Source identifiers:
641887
Deposit date:
2016-09-09

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