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DNA hairpins primarily promote duplex melting rather than inhibiting hybridization

Abstract:

The effect of secondary structure on DNA duplex formation is poorly understood. Using oxDNA, a nucleotide level coarse-grainedmodel of DNA, we study how hairpins influence the rate and reaction pathways of DNA hybridzation. We compare to experimental systems studied by Gao et al. and find that 3-base pair hairpins reduce the hybridization rate by a factor of 2, and 4-base pair hairpins by a factor of 10, compared to DNA with limited secondary structure, which is in good agreement with expe...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/nar/gkv582

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Theoretical Physics
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Physics
Sub department:
Theoretical Physics
Role:
Author
Journal:
Nucleic Acids Research
Volume:
43
Issue:
13
Pages:
6181-6190
Publication date:
2014-08-19
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Keywords:
Pubs id:
pubs:482452
UUID:
uuid:6753b187-c718-4f7d-9c71-7c77a8eb20c7
Local pid:
pubs:482452
Source identifiers:
482452
Deposit date:
2014-09-14

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