Journal article
A new phosphoramidite enables orthogonal double labelling to form combination oligonucleotide probes
- Abstract:
- Oligonucleotides labelled with thiazole orange intercalator and a reporter dye on the same thymine base have been synthesized. The key phosphoramidite (AP-C3 dT) contains an alkyne and amine, enabling dual orthogonal labelling of the nucleobase. Multiple monomers can be added to produce heavily functionalised oligonucleotides. In their DNA and 2′-OMe RNA formats these combination probes display high duplex stability and fluorescence when bound to complementary DNA and RNA
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Version of record, pdf, 949.7KB, Terms of use)
-
- Publisher copy:
- 10.1039/d2ob01899c
Authors
- Publisher:
- Royal Society of Chemistry
- Journal:
- Organic & Biomolecular Chemistry More from this journal
- Volume:
- 20
- Issue:
- 44
- Pages:
- 8618-8622
- Publication date:
- 2022-11-16
- DOI:
- EISSN:
-
1477-0539
- ISSN:
-
1477-0520
- Language:
-
English
- Keywords:
- Pubs id:
-
1301201
- Local pid:
-
pubs:1301201
- Source identifiers:
-
W4307777289
- Deposit date:
-
2026-04-29
- ARK identifier:
This ORA record was generated from metadata provided by an external service. It has not been edited by the ORA Team.
Terms of use
- Copyright date:
- 2022
- Licence:
- CC Attribution (CC BY)
If you are the owner of this record, you can report an update to it here: Report update to this record