Dissecting tunicamycin biosynthesis: a potent carbohydrate processing enzyme inhibitor

Thesis

Tunicamycin nucleoside antibiotics were the first known to target the formation of peptidoglycan precursor lipid I in bacterial cell wall biosynthesis. They have also been used extensively as inhibitors of protein N-glycosylation in eukaryotes, blocking the biogenesis of early intermediate dolichyl-pyrophosphoryl-N-acetylglucosamine. Despite their unusual structures and useful biological properties, little is known about their biosynthesis. Elucidating the metabolic pathway of tunicamyc...

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Authors: Wyszynski, F; Filip Wyszynski

• Publication date: 2010
• DOI: 10.5287/ora-1ak4wr51m
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: Oxford University, UK
• Language: English
• Keywords: Tunicamycin; antibiotic; biosynthesis; epimerase; natural product; glycosyltransferase; Streptomyces
• Subjects: Bioinformatics (biochemistry); Biosynthesis; Antibiotics; Glycobiology; Organic chemistry; Molecular genetics; Chemical biology; Organic synthesis; Enzymes