Thesis
Developing liquid chromatography-mass spectrometry strategies for investigating energy metabolism with application to isocitrate dehydrogenase mutations in cancer
- Alternative title:
- Development and application of methods for qualitative and quantitative analysis of TCA cycle metabolites using liquid chromatography coupled to mass spectrometry
- Abstract:
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Isocitrate dehydrogenases (IDHs) act in the tricarboxylic acid (TCA) cycle to catalyse the conversion of isocitrate to 2-oxoglutarate (2-OG) with concomitant production of NADH and/or NADPH. In humans, mutations causing IDH1 and IDH2 substitutions have been found during DNA sequencing of human glioblastomas. Some cancerassociated IDH mutations promote the reduction of 2-OG to give D-2-hydroxyglutarate (D-2-HG) a chiral, polar dicarboxylic acid.
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Investigations on the underlying links be...
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- Files:
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(Preview, Dissemination version, pdf, 8.2MB, Terms of use)
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Authors
Contributors
+ McCullagh, J
- Institution:
- University of Oxford
- Division:
- MPLS
- Department:
- Chemistry
- Role:
- Supervisor
- ORCID:
- 0000-0003-4733-1205
+ Schofield, C
- Institution:
- University of Oxford
- Division:
- MPLS
- Department:
- Chemistry
- Role:
- Supervisor
- ORCID:
- 0000-0002-0290-6565
- DOI:
- Type of award:
- DPhil
- Level of award:
- Doctoral
- Awarding institution:
- University of Oxford
- Language:
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English
- UUID:
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uuid:354009b1-30a5-43a0-bbf1-5907437ea53a
- Deposit date:
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2016-04-22
- ARK identifier:
Terms of use
- Copyright holder:
- Khalid Al-Qahtani
- Copyright date:
- 2015
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