Mechanistic studies on human prolyl hydroxylase domain 2

Thesis

2-Oxoglutarate (2OG) and Fe(II) dependent oxygenases, in particular human PHD1-3, catalyse the trans-4-prolyl hydroxylation of hypoxia inducible transcriptional factors (HIFs). HIFs are α,β-heterodimeric proteins that mediate cellular responses to hypoxia in animals. At sufficiently high concentrations of O₂, the PHDs catalyse hydroxylation of HIFα at two proline residues located in the C-terminal and N-terminal oxygen degradation domains (CODD and NODD). HIFα prolyl hydr...

Expand abstract

Authors: Fiorini, G

• DOI: 10.5287/ora-o81ekyqzy
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• Language: English
• Keywords: HIF prolyl hydroxylase; hypoxia inducible factor; hypoxia; oxygen sensing; 2OG oxygenases
• Subjects: Proline hydroxylase; Oxygen; Oxygenases