Studies on recently identified activities of the Jumonji-C oxygenase JMJD4 and the bacterial class D nucleophilic serine β-lactamases

Thesis

The Jumonji-C oxygenase JMJD4 and the bacterial class D nucleophilic serine β-lactamases (SBLs) have been recently found to catalyse unprecedented cyclisation reactions. Biochemical, biophysical, and inhibition studies were carried out to investigate the formation of β-lactones from carbapenems by class D SBLs, and the JMJD4 catalysed γ-lactam ring formation of eukaryotic release factor 1 (eRF1). Chapter 1 gives an introduction to the β-lactamase and oxygenase enzyme families to which the ...

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Authors: Aertker, K

• DOI: 10.5287/ora-pmqkwkqe0
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• Language: English
• Keywords: post-translational modification; mutagenesis, site-directed; kinetics; enzymology