Development of high throughput microscope mode secondary ion mass spectrometry imaging

Journal article

This paper describes the development and initial results from a secondary ion mass spectrometer coupled with microscope mode detection. Stigmatic ion microscope imaging enables us to decouple the primary ion (PI) beam focus from spatial resolution and is a promising route to attaining higher throughput for mass spectrometry imaging (MSI). Using a commercial C₆₀⁺ PI beam source, we can defocus the PI beam to give uniform intensity across a 2.5 mm² area. By coupling the beam with a position-sensitive spatial detector, we can achieve mass spectral imaging of positive and negative secondary ions (SIs), which we demonstrate using samples comprising metals and dyes. Our approach involves simultaneous desorption of ions across a large field of view, enabling mass spectral images to be recorded over an area of 2.5 mm² in a matter of seconds. Our instrument can distinguish spatial features with a resolution of better than 20 μm, and has a mass resolution of >500 at 500 u. There is considerable scope to improve this, and through simulations we estimate the future performance of the instrument.

Authors: Green, FM; Castellani, ME; Jia, Y; Eyres, A; Smith, N

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: American Chemical Society
• Journal: Journal of the American Society for Mass Spectrometry
• Volume: 34
• Issue: 7
• Pages: 1272-1282
• Publication date: 2023-06-15
• Acceptance date: 2023-05-31
• DOI: 10.1021/jasms.2c00371
• EISSN: 1879-1123
• ISSN: 1044-0305
• Pmid: 37317808
• Language: English
• Keywords: bioengineering; chemical sciences; physical chemistry; analytical chemistry