Journal article

A hitchhiker's guide to click-chemistry with nucleic acids

Abstract:: Click chemistry is an immensely powerful technique for the fast and efficient covalent conjugation of molecular entities. Its broad scope has positively impacted on multiple scientific disciplines, and its implementation within the nucleic acid field has enabled researchers to generate a wide variety of tools with application in biology, biochemistry, and biotechnology. Azide-alkyne cycloadditions (AAC) are still the leading technology among click reactions due to the facile modification and incorporation of azide and alkyne groups within biological scaffolds. Application of AAC chemistry to nucleic acids allows labeling, ligation, and cyclization of oligonucleotides efficiently and cost-effectively relative to previously used chemical and enzymatic techniques. In this review, we provide a guide to inexperienced and knowledgeable researchers approaching the field of click chemistry with nucleic acids. We discuss in detail the chemistry, the available modified-nucleosides, and applications of AAC reactions in nucleic acid chemistry and provide a critical view of the advantages, limitations, and open-questions within the field.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Fantoni, N. Z., El-Sagheer, A. H., & Brown, T. (2021). A hitchhiker's guide to click-chemistry with nucleic acids. Chemical Reviews, 121(12), 7122–7154.

MLA Style

Fantoni, NZ, et al. “A Hitchhiker's Guide to Click-Chemistry with Nucleic Acids.” Chemical Reviews, vol. 121, no. 12, 2021, pp. 7122–54.

Chicago Style

Fantoni, NZ, AH El-Sagheer, and T Brown. 2021. “A Hitchhiker's Guide to Click-Chemistry with Nucleic Acids.” Chemical Reviews 121 (12): 7122–54.
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Files:: Fantoni_et_al_2021_a_hitchhikers_guide.pdf

(Preview, Version of record, pdf, 6.8MB, Terms of use)

Publisher copy:: 10.1021/acs.chemrev.0c00928

Authors

+ Fantoni, NZ More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Chemistry
Role:: Author

+ El-Sagheer, AH More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Chemistry
Role:: Author

+ Brown, T More by this author

Institution:: University of Oxford
Department:: CHEMISTRY
Sub department:: Organic Chemistry
Role:: Author
ORCID:: 0000-0002-6538-3036

Publisher:: American Chemical Society
Journal:: Chemical Reviews More from this journal
Volume:: 121
Issue:: 12
Pages:: 7122–7154
Publication date:: 2021-01-14
Acceptance date:: 2020-12-10
DOI:: 10.1021/acs.chemrev.0c00928
EISSN:: 1520-6890
ISSN:: 0009-2665
Pmid:: 33443411

Language:: English
Keywords:: FFR
Pubs id:: 1156751
Local pid:: pubs:1156751
Deposit date:: 2021-03-09
ARK identifier:: ark:/29072/ora_1eafd1169c824e26a375ab5a1e0bfef5

Terms of use

Copyright holder:: Crown
Copyright date:: 2021
Rights statement:: © Crown 2021 . Published by American Chemical Society. This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.

Licence:: CC Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND)

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