Structural and biophysical studies of HIV Rev and HBV e-antigen

Thesis

Human immunodeficiency virus (HIV) Rev and Hepatitis B virus (HBV) e-antigen are both viral proteins that have key functions in their respective viral replication cycles. Both have evaded crystallization for decades due to their tendency to aggregate and/or form higher-order species. In this thesis the structure determination of HIV Rev and HBV e-antigen is presented—achieved via complexing with monoclonal antibody Fab fragments—and their structures are analysed.

HIV Rev is a small ...

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Authors: DiMattia, M

• Publication date: 2012
• DOI: 10.5287/ora-n65yrxay5
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• Language: English
• Keywords: structural biology; cryo-electron microscopy; X-ray crystallography
• Subjects: Immunology; Molecular biophysics (biochemistry); Biochemistry; Disease prevention; HIV/AIDS; Infectious diseases