- Abstract:
-
Robust and cost-effective genome editing in a diverse array of cells and model organisms is now possible thanks to the discovery of the RNA-guided endonucleases of the CRISPR-Cas system. The commonly used Cas9 of Streptococcus pyogenes shows high levels of activity but, depending on the application, has been associated with some shortcomings. Firstly, the enzyme has been shown to cause mutagenesis at genomic sequences resembling the target sequence. Secondly, the stringent requirement for a s...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's version
- Files:
-
-
(pdf, 1.2MB)
-
- Publisher copy:
- 10.1007/s00335-017-9697-4
-
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- Publisher:
- Springer Verlag Publisher's website
- Journal:
- Mammalian Genome Journal website
- Volume:
- 28
- Issue:
- 7-8
- Pages:
- 247–261
- Publication date:
- 2017-06-20
- Acceptance date:
- 2017-05-26
- DOI:
- EISSN:
-
1432-1777
- ISSN:
-
0938-8990
- Pubs id:
-
pubs:702021
- URN:
-
uri:037bb1dc-b798-4609-ab0a-da7c1f140ad8
- UUID:
-
uuid:037bb1dc-b798-4609-ab0a-da7c1f140ad8
- Local pid:
- pubs:702021
- Paper number:
- 7-8
- Language:
- English
- Keywords:
- Copyright holder:
- Cebrian-Serrano and Davies
- Copyright date:
- 2017
- Notes:
- © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.
Journal article
CRISPR-Cas orthologues and variants: optimizing the repertoire, specificity and delivery of genome engineering tools
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