Journal article
CRISPR-Cas orthologues and variants: optimizing the repertoire, specificity and delivery of genome engineering tools
- Abstract:
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Robust and cost-effective genome editing in a diverse array of cells and model organisms is now possible thanks to the discovery of the RNA-guided endonucleases of the CRISPR-Cas system. The commonly used Cas9 of Streptococcus pyogenes shows high levels of activity but, depending on the application, has been associated with some shortcomings. Firstly, the enzyme has been shown to cause mutagenesis at genomic sequences resembling the target sequence. Secondly, the stringent requirement for a s...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, pdf, 1.2MB)
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- Publisher copy:
- 10.1007/s00335-017-9697-4
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Authors
Funding
Bibliographic Details
- Publisher:
- Springer Verlag Publisher's website
- Journal:
- Mammalian Genome Journal website
- Volume:
- 28
- Issue:
- 7-8
- Pages:
- 247–261
- Publication date:
- 2017-06-20
- Acceptance date:
- 2017-05-26
- DOI:
- EISSN:
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1432-1777
- ISSN:
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0938-8990
- Source identifiers:
-
702021
Item Description
- Language:
- English
- Keywords:
- Pubs id:
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pubs:702021
- UUID:
-
uuid:037bb1dc-b798-4609-ab0a-da7c1f140ad8
- Local pid:
- pubs:702021
- Deposit date:
- 2017-06-27
Terms of use
- Copyright holder:
- Cebrian-Serrano and Davies
- Copyright date:
- 2017
- Notes:
- © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.
- Licence:
- CC Attribution (CC BY)
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