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Journal article

CRISPR-Cas orthologues and variants: optimizing the repertoire, specificity and delivery of genome engineering tools

Abstract:

Robust and cost-effective genome editing in a diverse array of cells and model organisms is now possible thanks to the discovery of the RNA-guided endonucleases of the CRISPR-Cas system. The commonly used Cas9 of Streptococcus pyogenes shows high levels of activity but, depending on the application, has been associated with some shortcomings. Firstly, the enzyme has been shown to cause mutagenesis at genomic sequences resembling the target sequence. Secondly, the stringent requirement for a s...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1007/s00335-017-9697-4

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Author
Publisher:
Springer Verlag Publisher's website
Journal:
Mammalian Genome Journal website
Volume:
28
Issue:
7-8
Pages:
247–261
Publication date:
2017-06-20
Acceptance date:
2017-05-26
DOI:
EISSN:
1432-1777
ISSN:
0938-8990
Source identifiers:
702021
Language:
English
Keywords:
Pubs id:
pubs:702021
UUID:
uuid:037bb1dc-b798-4609-ab0a-da7c1f140ad8
Local pid:
pubs:702021
Deposit date:
2017-06-27

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