Structure–function studies of the oxidoreductase bilirubin oxidase from Myrothecium verrucaria using an electrochemical quartz crystal microbalance with dissipation

Thesis

This thesis presents the development and redesign of a commercial electrochemical quartz crystal microbalance with dissipation (E–QCM–D). This was used to study factors affecting the efficiency of the four electron reduction catalysed by the fuel cell enzyme bilirubin oxidase from Myrothecium verrucaria immobilised on thiol modified gold surfaces.

Within this thesis, the E–QCM–D was used to show that application of a constant potential to bilirubin oxidase adsorbed to thiol...

Expand abstract

Authors: Singh, K

• Publication date: 2014
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• Language: English
• Keywords: adsorption; voltammetry; reduction; laccase; Electrochemical quartz-crystal microbalance; QCM; enzyme catalysis; surface chemistry; fuel cell; bilirubin oxidase; E-QCM; redox enzyme; oxygen reduction; gold electrodes; Quartz-crystal microbalance; electrochemistry
• Subjects: Physical & theoretical chemistry; Catalysis; Electrochemistry and electrolysis; Materials Sciences; Chemistry & allied sciences; Surface chemistry; Materials engineering; Biochemistry; Chemical kinetics; Computer aided molecular and material design; Organic chemistry; Surfaces; Biophysical chemistry; Surface analysis; Enzymes; Inorganic chemistry