Journal article
Unraveling Subcellular Ultrastructure with Cyclically Multiplexed Expansion Microscopy
- Abstract:
- Despite advances in fluorescence microscopy, spectral overlap and limited resolution hinder the dense mapping of the cellular ultrastructure. To overcome these challenges, we developed Cy-ExM, a high-plex imaging strategy that integrates optimized cryo-fixation for antigen preservation, expansion microscopy, and iterative immunofluorescence labeling. Using oblique plane microscopy, we perform three-dimensional super-resolution imaging of 20 biological targets encompassing the full cellular volume of individual mammalian cells.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 9.2MB, Terms of use)
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- Publisher copy:
- 10.64898/2026.01.01.697161
Authors
- Publisher:
- openRxiv
- Journal:
- bioRxiv More from this journal
- Publication date:
- 2026-01-02
- DOI:
- EISSN:
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2692-8205
- Language:
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English
- Keywords:
- Pubs id:
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2409307
- Local pid:
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pubs:2409307
- Source identifiers:
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W7117986318
- Deposit date:
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2026-04-21
- ARK identifier:
This ORA record was generated from metadata provided by an external service. It has not been edited by the ORA Team.
Terms of use
- Copyright date:
- 2026
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from openRxiv at https://doi.org/10.64898/2026.01.01.697161
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