Journal article
Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane
- Abstract:
- Lipid packing is a crucial feature of cellular membranes. Quantitative analysis of membrane lipid packing can be achieved using polarity sensitive probes whose emission spectrum depends on the lipid packing. However, detailed insights into the exact mechanisms that cause the changes in the spectra are necessary to interpret experimental fluorescence emission data correctly. Here, we analysed frequently used polarity sensitive probes, Laurdan and di-4-ANEPPDHQ, to test whether the underlying physical mechanisms of their spectral changes are the same and, thus, whether they report on the same physico-chemical properties of the cell membrane. Steady-state spectra as well as time-resolved emission spectra of the probes in solvents and model membranes revealed that they probe different properties of the lipid membrane. Our findings are important for the application of these dyes in cell biology.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.0MB, Terms of use)
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- Publisher copy:
- 10.1088/1361-6463/aa5dbc
Authors
+ Marie Curie Intra-European Fellowships
More from this funder
- Funding agency for:
- Sezgin, E
- Grant:
- MEMBRANE DYNAMICS
+ European Molecular Biology Organization
More from this funder
- Funding agency for:
- Sezgin, E
- Grant:
- MEMBRANE DYNAMICS
+ Czech Science Foundation
More from this funder
- Funding agency for:
- Amaro, M
- Hof, M
- Grant:
- P208/12/G016
- P208/12/G016
+ Deutsche Forschungsgemeinschaft
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- Grant:
- functionoftheperoxisomaltranslocon’
- Researchunit1905‘Structure
- Publisher:
- IOP Publishing
- Journal:
- Journal of Physics D: Applied Physics More from this journal
- Volume:
- 50
- Issue:
- 13
- Pages:
- 134004
- Publication date:
- 2017-03-07
- Acceptance date:
- 2017-02-02
- DOI:
- EISSN:
-
1361-6463
- ISSN:
-
0022-3727
- Keywords:
- Pubs id:
-
pubs:687132
- UUID:
-
uuid:ff80e6ac-bfad-4a3d-9118-116b44429540
- Local pid:
-
pubs:687132
- Source identifiers:
-
687132
- Deposit date:
-
2017-05-11
Terms of use
- Copyright holder:
- © 2017 IOP Publishing Ltd
- Copyright date:
- 2017
- Notes:
- Original content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
- Licence:
- CC Attribution (CC BY)
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