Assessment of diatom growth using fluorescence imaging

Conference item

Monitoring the growth of diatom microalgae requires sensitive, fast, and easy-to-use methods for selecting the most productive strains for further research and application. Here we propose a novel, rapid and non-destructive approach for monitoring the viability and growth of diatom colonies using the IVIS fluorescence imaging system. The fast-growing Achnanthidium sibiricum diatoms were cultivated in favorable laboratory conditions in a specially designed bioincubator for 100 days. The results showed that the diatoms reached the end of the exponential growth phase after 56 days of culture, after which there was a slight decrease in fluorescence intensity. This method relies on using chlorophyll a fluorescence without any sample preparation and has proved to be successful for monitoring algae growth in an incubator during long-term batch cultivation. This result paves the way towards controlled diatom cultivation aimed at collecting diatom-derived bioactive compounds for various purposes.

Authors: Cvjetinovic, J; Nozdriukhin, DV; Bedoshvili, YD; Salimon, AI; Korsunsky, AM

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: IOP Publishing
• Journal: Journal of Physics: Conference Series
• Volume: 1984
• Article number: 012017
• Publication date: 2021-09-13
• DOI: 10.1088/1742-6596/1984/1/012017
• EISSN: 1742-6596
• ISSN: 1742-6588
• Language: English
• Keywords: FFR