Calcium dynamics in dendrites of hippocampal CA1 interneurons in awake mice

Journal article

Hippocampal inhibitory interneurons exhibit a large diversity of dendritic Ca2+ mechanisms that are involved in the induction of Hebbian and anti-Hebbian synaptic plasticity. High resolution imaging techniques allowed examining somatic Ca2+ signals and, accordingly, the recruitment of hippocampal interneurons in awake behaving animals. However, little is still known about dendritic Ca2+ activity in interneurons during different behavioral states. Here, we used two-photon Ca2+ imaging in mouse hippocampal CA1 interneurons to reveal Ca2+ signal patterns in interneuron dendrites during animal locomotion and immobility. Despite overall variability in dendritic Ca2+ transients (CaTs) across different cells and dendritic branches, we report consistent behavior state-dependent organization of Ca2+ signaling in interneurons. As such, spreading regenerative CaTs dominated in dendrites during locomotion, whereas both spreading and localized Ca2+ signals were seen during immobility. Thus, these data indicate that while animal locomotion is associated with widespread Ca2+ elevations in interneuron dendrites that may reflect regenerative activity, local CaTs that may be related to synaptic activity become apparent during animal quiet state.

Authors: Francavilla, R; Villette, V; Martel, O; Topolnik, L

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Frontiers Media
• Journal: Frontiers in Cellular Neuroscience
• Volume: 13
• Issue: March 2019
• Article number: 98
• Publication date: 2019-03-15
• Acceptance date: 2019-02-27
• DOI: 10.3389/fncel.2019.00098
• ISSN: 1662-5102
• Keywords: FFR