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The protective role of DOT1L in UV-induced melanomagenesis

Abstract:
The DOT1L histone H3 lysine 79 (H3K79) methyltransferase plays an oncogenic role in MLL-rearranged leukemogenesis. Here, we demonstrate that, in contrast to MLL-rearranged leukemia, DOT1L plays a protective role in ultraviolet radiation (UVR)-induced melanoma development. Specifically, the DOT1L gene is located in a frequently deleted region and undergoes somatic mutation in human melanoma. Specific mutations functionally compromise DOT1L methyltransferase enzyme activity leading to reduced H3K79 methylation. Importantly, in the absence of DOT1L, UVR-induced DNA damage is inefficiently repaired, so that DOT1L loss promotes melanoma development in mice after exposure to UVR. Mechanistically, DOT1L facilitates DNA damage repair, with DOT1L-methylated H3K79 involvement in binding and recruiting XPC to the DNA damage site for nucleotide excision repair (NER). This study indicates that DOT1L plays a protective role in UVR-induced melanomagenesis.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41467-017-02687-7

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Role:
Author
ORCID:
0000-0003-4247-6245


Publisher:
Springer Nature
Journal:
Nature Communications More from this journal
Volume:
9
Issue:
1
Article number:
259
Publication date:
2018-01-17
Acceptance date:
2017-12-13
DOI:
ISSN:
2041-1723


Language:
English
Pubs id:
pubs:820637
UUID:
uuid:feeb4081-a35d-4096-a89e-d9a6e90fcca2
Local pid:
pubs:820637
Source identifiers:
820637
Deposit date:
2018-01-23
ARK identifier:

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