Chemotaxis of cell aggregates: morphology and dynamics of migrating active droplets

Journal article

Biological tissues have been observed to display emergent fluid-like properties, owing to physical interactions between cells. However, it remains unclear in general how these fluid-like properties affect tissue structure and function. Here, we are motivated by recent experiments in which cell aggregates were observed to behave as active droplets during collective migration along chemical gradients, or chemotaxis. To understand this process, we develop a minimal model of a growing thin active droplet driven by a self-generated chemical gradient. In broad agreement with the experiments, dynamic simulations reveal that chemotacting droplets exhibit proliferation-driven morphological transitions. To fully characterise these transitions, we perform a multiple scales analysis to show that the droplet dynamics follow a sequence of travelling wave solutions defined by a nonlinear eigenvalue problem parametrised by the slowly increasing droplet volume. Our analysis reveals that morphological transitions can occur continuously or through a discontinuous bifurcation. Further asymptotic analysis of the travelling wave problem reveals that these morphological transitions arise from exponentially small asymptotic terms that originate from the rear and front contact lines. Moreover, we show that the nature of the transitions is fully determined by two key dimensionless parameters, which quantify the internal stress balance within the droplet and the strength of the coupling between the droplet migration dynamics and the external chemical field. Overall, our results provide a complete characterisation of the morphodynamics of a class of migrating active thin droplets, with implications in a range of biological systems where cell aggregates exhibit fluid-like behaviour.

Authors: Celora, G; Walker, B; Dalwadi, M; Pearce, P

• Publication status: Accepted
• Peer review status: Peer reviewed
• Publisher: Cambridge University Press
• Journal: Journal of Fluid Mechanics
• Acceptance date: 2026-09-14
• EISSN: 1469-7645
• ISSN: 0022-1120
• Language: English
• Keywords: exponential asymptotics; active fluids; thin films; travelling wave analysis; matched asymptotic expansions