Journal article
Development of a monoclonal anti-ADAMTS-5 antibody that specifically blocks the interaction with LRP1
- Abstract:
- The potent aggrecanase ADAMTS-5 is constitutively secreted by chondrocytes, but it is rapidly endocytosed in normal cartilage via the cell surface endocytic receptor LRP1. Therefore it is difficult to detect the total ADAMTS-5 activity produced. In this study, we isolated a monoclonal anti-ADAMTS-5 antibody 1B7 that blocks LRP1-mediated internalization without affecting the aggrecanolytic activity. Addition of 1B7 to cultured human chondrocytes revealed the full aggrecanolytic activity of ADAMTS-5 generated by the cells. 1B7 is a useful tool to estimate the ADAMTS-5 activity and to identify its potential roles in the tissues.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.4MB, Terms of use)
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- Publisher copy:
- 10.1080/19420862.2017.1304341
Authors
+ National Institute of Arthritis and Musculoskeletal and Skin Diseases
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- Grant:
- AR40994
+ Arthritis Research UK
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- Grant:
- 20563
- Center for Osteoarthritis Pathogenesis (20205
- Publisher:
- Taylor and Francis
- Journal:
- mAbs More from this journal
- Volume:
- 9
- Issue:
- 4
- Pages:
- 595-602
- Publication date:
- 2017-03-17
- Acceptance date:
- 2017-03-05
- DOI:
- EISSN:
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1942-0870
- ISSN:
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1942-0862
- Language:
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English
- Keywords:
- Pubs id:
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pubs:686825
- UUID:
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uuid:fe0e3165-3193-4fdd-93a8-537794f3a543
- Local pid:
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pubs:686825
- Source identifiers:
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686825
- Deposit date:
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2017-04-27
- ARK identifier:
Terms of use
- Copyright holder:
- Santamaria et al
- Copyright date:
- 2017
- Notes:
-
Published with license by Taylor and Francis Group, LLC © Salvatore Santamaria, Oleg Fedorov, John McCafferty, Gillian Murphy, Jayesh Dudhia, Hideaki Nagase, and Kazuhiro Yamamoto.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
- Licence:
- CC Attribution (CC BY)
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