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Dihydrogen‐driven NADPH recycling in imine reduction and P450‐catalyzed oxidations mediated by an engineered O2‐tolerant hydrogenase

Abstract:

The O2‐tolerant NAD+‐reducing hydrogenase (SH) from Ralstonia eutropha (Cupriavidus necator) has already been applied in vitro and in vivo for H2‐driven NADH recycling in coupled enzymatic reactions with various NADH‐dependent oxidoreductases. To expand the scope for application in NADPH‐dependent biocatalysis, we introduced changes in the NAD+‐binding pocket of the enzyme by rational mutagenesis, and generated a variant with significantly higher affinity for NADP+ than for the natural substr...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1002/cctc.202000763

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Inorganic Chemistry
Role:
Author
ORCID:
0000-0001-5855-651X
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Inorganic Chemistry
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Inorganic Chemistry
Role:
Author
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Publisher:
Wiley Publisher's website
Journal:
ChemCatChem Journal website
Volume:
12
Issue:
19
Pages:
4853-4861
Publication date:
2020-08-10
Acceptance date:
2020-06-16
DOI:
EISSN:
1867-3899
ISSN:
1867-3880
Language:
English
Keywords:
Pubs id:
1112575
Local pid:
pubs:1112575
Deposit date:
2020-06-17

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