Journal article
Dihydrogen‐driven NADPH recycling in imine reduction and P450‐catalyzed oxidations mediated by an engineered O2‐tolerant hydrogenase
- Abstract:
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The O2‐tolerant NAD+‐reducing hydrogenase (SH) from Ralstonia eutropha (Cupriavidus necator) has already been applied in vitro and in vivo for H2‐driven NADH recycling in coupled enzymatic reactions with various NADH‐dependent oxidoreductases. To expand the scope for application in NADPH‐dependent biocatalysis, we introduced changes in the NAD+‐binding pocket of the enzyme by rational mutagenesis, and generated a variant with significantly higher affinity for NADP+ than for the natural substr...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, 1.3MB)
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- Publisher copy:
- 10.1002/cctc.202000763
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Authors
Bibliographic Details
- Publisher:
- Wiley Publisher's website
- Journal:
- ChemCatChem Journal website
- Volume:
- 12
- Issue:
- 19
- Pages:
- 4853-4861
- Publication date:
- 2020-08-10
- Acceptance date:
- 2020-06-16
- DOI:
- EISSN:
-
1867-3899
- ISSN:
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1867-3880
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
1112575
- Local pid:
- pubs:1112575
- Deposit date:
- 2020-06-17
Terms of use
- Copyright holder:
- Preissler, J et al.
- Copyright date:
- 2020
- Rights statement:
- © 2020 The Authors. Published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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