Guidelines for DNA recombination and repair studies: mechanistic assays of DNA repair processes

Journal article

Genomes are constantly in flux, undergoing changes due to recombination, repair and mutagenesis. In vivo, many of such changes are studies using reporters for specific types of changes, or through cytological studies that detect changes at the single-cell level. Single molecule assays, which are reviewed here, can detect transient intermediates and dynamics of events. Biochemical assays allow detailed investigation of the DNA and protein activities of each step in a repair, recombination or mutagenesis event. Each type of assay is a powerful tool but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.

Authors: Klein, HL; Ang, KKH; Arkin, MR; Beckwitt, EC; Chang, Y-H

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Shared Science Publishers
• Journal: Microbial Cell
• Volume: 6
• Issue: 1
• Pages: 65 -101
• Publication date: 2019-01-07
• Acceptance date: 2018-10-31
• DOI: 10.15698/mic2019.01.665
• EISSN: 2311-2638
• Pmid: 30652106
• Language: English
• Keywords: FRET; double strand break repair; homologous recombination; synthesis-dependent strand annealing; single-particle tracking; super resolution; chromosome rearrangements; DSBs; transcription coupled repair; PALM; recombinase filament assembly; structure-selective endonucleases; gross chromosome rearrangements; photoactivated fluorescent proteins; endonuclease protection assay; DNA helicases; genome instability; chromatin dynamics; nucleotide excision repair; DNA resection; DNA repair centers; DNA repair synthesis; DNA breaks; single-molecule; nonhomologous end joining; mismatch repair; crossovers; fluorescent proteins