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Journal article

Value of Plasmodium falciparum histidine-rich protein 2 level and malaria retinopathy in distinguishing cerebral malaria from other acute encephalopathies in Kenyan children

Abstract:

Background. The diagnosis of cerebral malaria is problematic in malaria-endemic areas because encephalopathy in patients with parasitemia may have another cause. Abnormal retinal findings are thought to increase the specificity of the diagnosis, and the level of histidine-rich protein 2 (HRP2) may reflect the parasite biomass.

Methods. We examined the retina and measured plasma HRP2 levels in children with acute nontraumatic encephalopathy in Kenya. Logistic regression, with HRP2 level as an independent variable and World Health Organization–defined cerebral malaria and/or retinopathy as the outcome, was used to calculate malaria-attributable fractions (MAFs) and retinopathy-attributable fractions (RAFs).

Results. Of 270 children, 140 (52%) had peripheral parasitemia, 80 (30%) had malaria retinopathy, and 164 (61%) had an HRP2 level of >0 U/mL. During 2006–2011, the incidence of HRP2 positivity among admitted children declined by 49 cases per 100 000 per year (a 78% reduction). An HRP2 level of >0 U/mL had a MAF of 93% for cerebral malaria, with a MAF of 97% observed for HRP2 levels of ≥10 U/mL (the level of the best combined sensitivity and specificity). HRP2 levels of >0 U/mL had a RAF of 77% for features of retinopathy combined, with the highest RAFs for macular whitening (99%), peripheral whitening (98%), and hemorrhages (90%).

Conclusion. HRP2 has a high attributable fraction for features of malarial retinopathy, supporting its use in the diagnosis of cerebral malaria. HRP2 thresholds improve the specificity of the definition.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/infdis/jit500

Authors

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Institution:
University of Oxford
Division:
MSD
Department:
Psychiatry
Role:
Author


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Funder identifier:
https://ror.org/029chgv08
Grant:
083744
099782
079082


Publisher:
Oxford University Press
Journal:
Journal of Infectious Diseases More from this journal
Volume:
209
Issue:
4
Pages:
600-609
Publication date:
2013-09-16
Acceptance date:
2013-08-28
DOI:
EISSN:
1537-6613
ISSN:
0022-1899


Language:
English
Keywords:
Pubs id:
pubs:429343
UUID:
uuid:faafea03-02b2-488e-a915-8e11efad4eb6
Local pid:
pubs:429343
Source identifiers:
429343
Deposit date:
2013-11-16
ARK identifier:

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