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Cultured ELISpot assay to investigate dengue virus specific T-cell responses

Abstract:

The cultured Enzyme-Linked ImmunoSpot (ELISpot) assay is a functional T cell assay, which is commonly used to assess virus-specific T cell responses. The use of an in vitro expansion step before the ELISpot distinguishes such “cultured” ELISpots from “ex vivo” ELISpots. Cultured ELISpots have the advantage that lower frequency responses can be analyzed compared to ex vivo ELISpots, but do carry the associated potential distortions of the expansion phase. Cultured ELISpot assays are of value t...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Authors


Jeewandara, C More by this author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Subgroup:
RDM - Investigative Medicine Division
ORCID:
0000-0002-3097-045X
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
RDM
Subgroup:
Weatherall Insti. of Molecular Medicine
Publisher:
Humana Press Publisher's website
Volume:
1808
Pages:
165-171
Series:
Methods in Molecular Biology
Host title:
Handbook of ELISPOT
Publication date:
2018-06-29
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
Pubs id:
pubs:869709
URN:
uri:faac72bf-5cb2-4616-83aa-48302bb5e99f
UUID:
uuid:faac72bf-5cb2-4616-83aa-48302bb5e99f
Local pid:
pubs:869709
ISBN:
978-1-4939-8566-1

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