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CMV viraemia is associated with mortality among children with HIV starting antiretroviral therapy in sub-Saharan Africa

Abstract:
Background:
Cytomegalovirus (CMV) co-infection is associated with mortality in adults with HIV, but whether CMV is associated with mortality in children with HIV remains uncertain.
Methods:
In 498 children (median age 6.3 years, interquartile range 2.3-9.6) enrolled in the ARROW trial (ISRCTN24791884) in Uganda (336/498) and Zimbabwe (162/498) selected using a case-cohort design, CMV was quantified using real-time polymerase chain reaction at initiation of non-nucleotide reverse transcriptase inhibitor-based antiretroviral therapy (ART), 12-weeks post-ART, and 84-weeks post-ART. Associations between baseline CMV viraemia and mortality were evaluated using multivariable models, adjusting for baseline HIV viral load, CD4+ percentage, and IL-6 concentrations.
Results:
Baseline CMV viraemia was associated with mortality, with relationships differing by country and assay. In Zimbabwe (assay limit of detection 20 copies/mL), 119/162 (73%) children had detectable CMV, and each log10 higher CMV viral load was associated with over 2-fold higher mortality (adjusted hazard ratio (aHR)=2.74; 95% confidence interval (CI) 1.57-4.77). In Uganda (assay limit of detection 120 copies/mL), 89/336 (26%) children had detectable CMV viraemia, which was associated with 3-fold higher mortality compared to undetectable CMV (aHR=3.15; 95%CI 1.11-8.93). In a subset of 48 children with immunophenotyping data, we found no evidence that CMV was associated with immune activation.
Conclusions:
CMV viraemia is independently associated with mortality in children with HIV starting ART in sub-Saharan Africa. Future studies should define the underlying mechanisms and evaluate whether suppressing CMV viraemia reduces mortality in children with HIV.
Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1093/cid/ciag354

Authors


More from this funder
Funder identifier:
https://ror.org/029chgv08
Grant:
108065/Z/15/Z
More from this funder
Funder identifier:
https://ror.org/03x94j517
Grant:
MC_UU_00004/03
G0600344-E01/1
G0600344
G1001190
G0300400
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Funder identifier:
https://ror.org/0187kwz08
Grant:
NIHR305856


Publisher:
Oxford University Press
Journal:
Clinical Infectious Diseases More from this journal
Article number:
ciag354
Publication date:
2026-06-09
Acceptance date:
2026-04-11
DOI:
EISSN:
1537-6591
ISSN:
1058-4838


Language:
English
Keywords:
Pubs id:
2405450
Local pid:
pubs:2405450
Deposit date:
2026-04-16
ARK identifier:

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