Detergent-free mass spectrometry of membrane protein complexes.

Journal article

We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent.

Authors: Hopper, J; Yu, Y; Li, D; Raymond, A; Bostock, M

• Publication status: Published
• Journal: Nature methods
• Volume: 10
• Issue: 12
• Pages: 1206-1208
• Publication date: 2013-12-01
• DOI: 10.1038/nmeth.2691
• EISSN: 1548-7105
• ISSN: 1548-7091
• Language: English
• Keywords: Green Fluorescent Proteins; Escherichia coli Proteins; Sensory Rhodopsins; Mass Spectrometry; Plasmids; Halobacteriaceae; Symporters; Spectrometry, Mass, Electrospray Ionization; Monosaccharide Transport Proteins; Detergents; Magnetic Resonance Spectroscopy; Nanoparticles; Diacylglycerol Kinase; Micelles; Membrane Proteins; Escherichia coli; Diffusion; Microscopy, Electron, Transmission; Lipids