Imaging real-time HIV-1 virion fusion with FRET-based biosensors

Jones, DM; Padilla-Parra, S

Journal article

Imaging real-time HIV-1 virion fusion with FRET-based biosensors

Abstract:: ABSTRACT A major focus of current biological studies is to fill the knowledge gaps between cell, tissue and organism scales. To this end, a wide array of contemporary optical analytical tools enable multiparameter quantitative imaging of live and fixed cells, three-dimensional (3D) systems, tissues, organs and organisms in the context of their complex spatiotemporal biological and molecular features. In particular, the modalities of luminescence lifetime imaging, comprising fluorescence lifetime imaging (FLI) and phosphorescence lifetime imaging microscopy (PLIM), in synergy with Förster resonance energy transfer (FRET) assays, provide a wealth of information. On the application side, the luminescence lifetime of endogenous molecules inside cells and tissues, overexpressed fluorescent protein fusion biosensor constructs or probes delivered externally provide molecular insights at multiple scales into protein–protein interaction networks, cellular metabolism, dynamics of molecular oxygen and hypoxia, physiologically important ions, and other physical and physiological parameters. Luminescence lifetime imaging offers a unique window into the physiological and structural environment of cells and tissues, enabling a new level of functional and molecular analysis in addition to providing 3D spatially resolved and longitudinal measurements that can range from microscopic to macroscopic scale. We provide an overview of luminescence lifetime imaging and summarize key biological applications from cells and tissues to organisms.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Jones, D. M., & Padilla-Parra, S. (2015). Imaging real-time HIV-1 virion fusion with FRET-based biosensors. Scientific Reports, 5(1), 13449–13449.

MLA Style

Jones, DM, and S Padilla-Parra. “Imaging Real-Time HIV-1 Virion Fusion with FRET-Based Biosensors.” Scientific Reports, vol. 5, no. 1, 2015, pp. 13449–49.

Chicago Style

Jones, DM, and S Padilla-Parra. 2015. “Imaging Real-Time HIV-1 Virion Fusion with FRET-Based Biosensors.” Scientific Reports 5 (1): 13449–49.
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