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Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

Abstract:
Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.
Publication status:
Published
Peer review status:
Peer reviewed

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Files:
Publisher copy:
10.1039/D2CC04823J

Authors


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Role:
Author
ORCID:
0000-0002-6151-9151
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Role:
Author
ORCID:
0000-0002-5880-5245
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Role:
Author
ORCID:
0000-0002-0039-4762
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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
OCDEM
Research group:
NIHR Oxford Biomedical Research Centre
Oxford college:
Green Templeton College
Role:
Author
ORCID:
0000-0002-8641-8568


Publisher:
Royal Society of Chemistry
Journal:
Chemical Communications More from this journal
Volume:
58
Issue:
99
Pages:
13724-13727
Publication date:
2022-11-21
Acceptance date:
2022-11-09
DOI:
EISSN:
1364-548X
ISSN:
1359-7345


Language:
English
Keywords:
Pubs id:
1300805
Local pid:
pubs:1300805
Deposit date:
2022-11-09

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