The stem cell revolution: on the role of CD164 as a human stem cell marker

Watt, SM; Bühring, H-J; Simmons, PJ; Zannettino, AWC

Journal article

The stem cell revolution: on the role of CD164 as a human stem cell marker

Abstract:: Embryogenesis is a critical programming period, as environmental factors can modulate the physiology of organisms. In birds, incubation temperature has been widely studied as an inducer of genetic responses that influence embryonic development and response to post- hatch challenges. In this context, the present study aimed to investigate the effects of embryonic thermal manipulation on the immune response of Japanese quails (Coturnix coturnix japonica) challenged with lipopolysaccharide (LPS) after hatching, using transcriptome analysis (RNA- seq). For this purpose, an experiment was carried out in a 2x2 factorial design, with two incubation temperatures: control temperature (CTL - 37.5°C) and high temperature (AT - 39°C) and two inoculation statuses: quails inoculated with LPS (LPS) and quails not inoculated with 49 LPS (SLPS). Eggs remained at the respective treatment temperatures throughout the incubation period, and 25 days after hatching, chicks were challenged with LPS or saline (placebo). After inoculation, jejunal tissue was collected for gene expression analysis via RNA-seq. Incubation temperature significantly reduced (p0.05) the morphological performance of surviving embryos. LPS induced significant changes (p0,05) o desempenho morfológico dos embriões sobreviventes. O LPS induziu alterações significavas (p<0,05) no baço, indicando ativação do sistema imunológico. A análise transcriptômica revelou genes diferencialmente expressos (p-20 ajustado<0,05) associados a processos metabólicos, resposta imune e estresse oxidativo, com padrões distintos entre os contrastes avaliados (CTL+LPS vs CTL+SLPS e AT+LPS vs 22 AT+SLPS). Em condição controle, o LPS induziu a expressão diferencial de genes, destacando IL1R2, IL18BP e S100A12, relacionados à modulação inflamatória, integridade da barreira mucosa e metabolismo energético. Em aves incubadas sob alta temperatura, o LPS 25 desencadeou amplas alterações, com ativação de vias associadas à inflamação, homeostase lipídica, controle proteico, metabolismo energético (glicólise, pentoses e ciclo de Krebs) e tráfego vesicular, enquanto suprimia genes cruciais para imunidade adaptativa, angiogênese, diferenciação celular e regulação epigenética. Um conjunto conservado de genes inflamatórios (MMP7, PI3, SLC6A14) foi regulado (p-ajustado<0,05) em ambas as condições térmicas, sugerindo ativação de mecanismos centrais de defesa. Conclui-se que a alta temperatura durante a incubação compromete a eclosão, mas não o fenótipo da progênie; contudo, modula de forma profunda a resposta molecular ao LPS, alterando vias inflamatórias, metabólicas e regenerativas, o que pode impactar a adaptação intestinal a estresses futuros.São Cristóvã

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Watt, S. M., Bühring, H.-J., Simmons, P. J., & Zannettino, A. W. C. (2021). The stem cell revolution: on the role of CD164 as a human stem cell marker. Npj Regenerative Medicine, 6(1), 33–33.

MLA Style

Watt, SM, et al. “The Stem Cell Revolution: on the Role of CD164 as a Human Stem Cell Marker.” Npj Regenerative Medicine, vol. 6, no. 1, 2021, pp. 33–33.

Chicago Style

Watt, SM, H-J Bühring, PJ Simmons, and AWC Zannettino. 2021. “The Stem Cell Revolution: on the Role of CD164 as a Human Stem Cell Marker.” Npj Regenerative Medicine 6 (1): 33–33.
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