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Investigating time-independent and time-dependent diffusion phenomena using steady-state diffusion MRI

Abstract:

Diffusion MRI is a leading method to non-invasively characterise brain tissue microstructure across multiple domains and scales. Diffusion-weighted steady-state free precession (DW-SSFP) is an established imaging sequence for post-mortem MRI, addressing the challenging imaging environment of fixed tissue with short T2 and low diffusivities. However, a current limitation of DW-SSFP is signal interpretation: it is not clear what diffusion ‘regime’ the sequence probes and therefore its potential to characterise tissue microstructure. Building on Extended Phase Graphs (EPG), I establish two alternative representations of the DW-SSFP signal in terms of (1) conventional b-values (time-independent diffusion) and (2) encoding power-spectra (time-dependent diffusion). The proposed representations provide insights into how different parameter regimes and gradient waveforms impact the diffusion sensitivity of DW-SSFP. I subsequently introduce an approach to incorporate existing biophysical models into DW-SSFP without the requirement of extensive derivations, with time dependence estimated via a Gaussian phase approximation representation of the DW-SSFP signal. Investigations incorporating free-diffusion and tissue-relevant microscopic restrictions (cylinder of varying radius) give excellent agreement to complementary analytical models and Monte Carlo simulations. Experimentally, the time-independent representation is used to derive Tensor and proof-of-principle NODDI estimates in a whole human post-mortem brain. A final SNR-efficiency investigation demonstrates the theoretical potential of DW-SSFP for ultra-high field microstructural imaging.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41598-025-87377-x

Authors

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Institution:
University of Oxford
Division:
MSD
Department:
Clinical Neurosciences
Role:
Author
ORCID:
0000-0003-2095-8665


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Funder identifier:
https://ror.org/029chgv08
Grant:
222829/Z/21/Z


Publisher:
Springer Nature
Journal:
Scientific Reports More from this journal
Volume:
15
Issue:
1
Article number:
3580
Publication date:
2025-01-28
Acceptance date:
2025-01-20
DOI:
EISSN:
2045-2322


Language:
English
Pubs id:
2081430
Local pid:
pubs:2081430
Deposit date:
2025-01-29
ARK identifier:

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