Journal article icon

Journal article

Alteration of the co-substrate selectivity of deacetoxycephalosporin C synthase. The role of arginine 258.

Abstract:

Deacetoxycephalosporin C synthase is an iron(II) 2-oxoglutaratedependent oxygenase that catalyzes the oxidative ring-expansion of penicillin N to deacetoxycephalosporin C. The wild-type enzyme is only able to efficiently utilize 2-oxoglutarate and 2-oxoadipate as a 2-oxoacid co-substrate. Mutation of arginine 258, the side chain of which forms an electrostatic interaction with the 5-carboxylate of the 2-oxoglutarate co-substrate, to a glutamine residue reduced activity to about 5% of the wild...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1074/jbc.m100085200

Authors


Clifton, IJ More by this author
Expand authors...
Journal:
The Journal of biological chemistry
Volume:
276
Issue:
21
Pages:
18290-18295
Publication date:
2001-05-05
DOI:
EISSN:
1083-351X
ISSN:
0021-9258
URN:
uuid:f8904bd0-8b4b-46c2-b65a-75e8eecaa764
Source identifiers:
31855
Local pid:
pubs:31855

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP