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Ion mobility-mass spectrometry shows stepwise protein unfolding under alkaline conditions

Abstract:

Although native mass spectrometry is widely applied to monitor chemical or thermal protein denaturation, it is not clear to what extent it can inform about alkali-induced unfolding. Here, we probe the relationship between solution- and gas-phase structures of proteins under alkaline conditions. Native ion mobility-mass spectrometry reveals that globular proteins are destabilized rather than globally unfolded, which is supported by solution studies, providing detailed insights into alkali-indu...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1039/d0cc08135c

Authors


Publisher:
Royal Society of Chemistry
Journal:
Chemical Communications More from this journal
Volume:
57
Issue:
12
Pages:
1450-1453
Publication date:
2021-01-06
Acceptance date:
2021-01-06
DOI:
EISSN:
1364-548X
ISSN:
1359-7345
Language:
English
Keywords:
Pubs id:
1153041
Local pid:
pubs:1153041
Deposit date:
2021-01-12

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