Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer.

Journal article

A synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3gamma. A single aptamer, which showed similar affinities (K(D) approximately 10(-8)M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep.

Authors: Stevenson, R; Baxter, H; Aitken, A; Brown, T; Baxter, R

• Publication status: Published
• Journal: Bioorganic chemistry
• Volume: 36
• Issue: 5
• Pages: 215-219
• Publication date: 2008-10-01
• DOI: 10.1016/j.bioorg.2007.12.005
• EISSN: 1090-2120
• ISSN: 0045-2068
• Language: English
• Keywords: 14-3-3 Proteins; Protein Binding; DNA, Single-Stranded; DNA; Cloning, Molecular; Animals; Chromatography, Affinity; Aptamers, Nucleotide; Base Sequence; Oligonucleotides; Sheep; Humans; SELEX Aptamer Technique; Molecular Sequence Data; Recombinant Proteins; Scrapie