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Ex vivo expansion of hematopoietic stem cells derived from umbilical cord blood in rotating wall vessel.

Abstract:

Expansion of umbilical cord blood mononuclear cells (UCB MNCs) was carried out in a rotating wall vessel (RWV) bioreactor and tissue culture flasks (T-flasks) in serum-containing medium supplemented with relatively low doses of purified recombinant human cytokines (5.33 ng/ml IL-3, 16 ng/ml SCF, 3.33 ng/ml G-CSF, 2.13 ng/ml GM-CSF, 7.47 ng/ml FL and 7.47 ng/ml TPO) for 8 days. The cell density, pH and osmolality of the culture medium in the two culture systems were measured every 24h. Flow cy...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MPLS, Engineering Science
Role:
Author
Journal:
Journal of biotechnology
Volume:
124
Issue:
3
Pages:
592-601
Publication date:
2006-07-05
DOI:
EISSN:
1873-4863
ISSN:
0168-1656
URN:
uuid:f84c816b-0c67-4c4f-8857-b912b4a2449d
Source identifiers:
62031
Local pid:
pubs:62031

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