- Abstract:
-
Expansion of umbilical cord blood mononuclear cells (UCB MNCs) was carried out in a rotating wall vessel (RWV) bioreactor and tissue culture flasks (T-flasks) in serum-containing medium supplemented with relatively low doses of purified recombinant human cytokines (5.33 ng/ml IL-3, 16 ng/ml SCF, 3.33 ng/ml G-CSF, 2.13 ng/ml GM-CSF, 7.47 ng/ml FL and 7.47 ng/ml TPO) for 8 days. The cell density, pH and osmolality of the culture medium in the two culture systems were measured every 24h. Flow cy...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/j.jbiotec.2006.01.020
-
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- Journal:
- Journal of biotechnology
- Volume:
- 124
- Issue:
- 3
- Pages:
- 592-601
- Publication date:
- 2006-07-05
- DOI:
- EISSN:
-
1873-4863
- ISSN:
-
0168-1656
- URN:
-
uuid:f84c816b-0c67-4c4f-8857-b912b4a2449d
- Source identifiers:
-
62031
- Local pid:
- pubs:62031
- Copyright date:
- 2006
Journal article
Ex vivo expansion of hematopoietic stem cells derived from umbilical cord blood in rotating wall vessel.
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