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Journal article

A gentle method for preparing cyto- and nucleo-skeletons and associated chromatin.

Abstract:

We describe a method for permeabilizing and extracting cells that preserves both structure and function whilst allowing the cell derivatives to be handled freely. Cells are encapsulated in microbeads of agarose; the coat of agarose, which is freely permeable to small molecules, forms a protective layer around fragile cell constituents. Cells are then permeabilized by the non-ionic detergent Triton X-100 or antibody and complement in a buffer whose ionic composition mimics that of the cytoplas...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Journal of cell science More from this journal
Volume:
90 ( Pt 3)
Pages:
365-378
Publication date:
1988-07-01
EISSN:
1477-9137
ISSN:
0021-9533
Language:
English
Keywords:
Pubs id:
pubs:20608
UUID:
uuid:f822b5a8-1cd2-453f-8bb1-3f7fde42819c
Local pid:
pubs:20608
Source identifiers:
20608
Deposit date:
2012-12-19

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