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A gentle method for preparing cyto- and nucleo-skeletons and associated chromatin.

Abstract:

We describe a method for permeabilizing and extracting cells that preserves both structure and function whilst allowing the cell derivatives to be handled freely. Cells are encapsulated in microbeads of agarose; the coat of agarose, which is freely permeable to small molecules, forms a protective layer around fragile cell constituents. Cells are then permeabilized by the non-ionic detergent Triton X-100 or antibody and complement in a buffer whose ionic composition mimics that of the cytoplas...

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Publication status:
Published

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Authors


Jackson, DA More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Journal:
Journal of cell science
Volume:
90 ( Pt 3)
Pages:
365-378
Publication date:
1988-07-05
EISSN:
1477-9137
ISSN:
0021-9533
URN:
uuid:f822b5a8-1cd2-453f-8bb1-3f7fde42819c
Source identifiers:
20608
Local pid:
pubs:20608

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