Potent triple helix stabilization by 5',3'-modified triplex-forming oligonucleotides.

Journal article

Anthraquinone and pyrene analogues attached to the 3' and/or 5' termini of triplex-forming oligonucleotides (TFOs) by various linkers increased the stability of parallel triple helices. The modifications are simple to synthesize and can be introduced during standard solid-phase oligonucleotide synthesis. Potent triplex stability was achieved by using doubly modified TFOs, which in the most favourable cases gave an increase in melting temperature of 30 degrees C over the unmodified counterparts and maintained their selectivity for the correct target duplex. Such TFOs can produce triplexes with melting temperatures of 40 degrees C at pH 7 even though they do not contain any triplex-stabilizing base analogues. These studies have implications for the design of triplex-forming oligonucleotides for use in biology and nanotechnology.

Authors: Ben Gaied, N; Zhao, Z; Gerrard, SR; Fox, K; Brown, T

• Publication status: Published
• Journal: Chembiochem : a European journal of chemical biology
• Volume: 10
• Issue: 11
• Pages: 1839-1851
• Publication date: 2009-07-01
• DOI: 10.1002/cbic.200900232
• EISSN: 1439-7633
• ISSN: 1439-4227
• Language: English
• Keywords: Oligonucleotides; Transition Temperature; Pyrenes; Ultraviolet Rays; DNA; Base Sequence; Anthraquinones; Amines; Nucleic Acid Conformation