The HLA-A,B,C genotype of the class I negative cell line Daudi reveals novel HLA-A and -B alleles.

Journal article

Daudi, a lymphoblastoid B cell line derived from an African Burkitt lymphoma does not express HLA-A,B,C antigens at the cell surface. Although HLA-A,B,C heavy chains are made normally they do not assemble into functional molecules because beta 2-microglobulin is absent. Previous serological analysis of somatic cell hybrids indicated that the HLA haplotypes of Daudi encoded HLA-A1, A10(A26), B17, and B16(38) antigens. Here we describe the application of molecular methods: ARMS-PCR, cDNA cloning and sequencing, immunoprecipitation and gel electrophoresis, to define the class I genotype of the Daudi cell line which is HLA-A*0102, A*6601, B*5801, B*5802, Cw*0302 and Cw*0602. With the exception of the B38 antigen, which is not a product of the alleles defined, the genotype is consistent with the serological description. Two previously undiscovered alleles emerged from this analysis: A*0102 and B*5802. The A*0102 allele differs from A*0101 by 5 nucleotide substitutions within exon 2 where it has a motif shared with A*30 alleles; the B*5802 allele differs from B*5801 by 3 substitutions in exon 3 where it has a motif shared with B*14 alleles. Subtyping HLA-A1 alleles showed A*0102 was well represented amongst individuals typed serologically as A1 in an African population but was absent from caucasoids. B*5802 has been found in a second individual. Thus the novel A and B alleles are not specific to the Daudi tumor. Overall, this analysis of a single East African cell illustrates the power of molecular methods to define new class I HLA alleles in non-caucasoid populations.

Authors: Browning, M; Madrigal, J; Krausa, P; Kowalski, H; Allsopp, C

• Publication status: Published
• Journal: Tissue antigens
• Volume: 45
• Issue: 3
• Pages: 177-187
• Publication date: 1995-03-01
• DOI: 10.1111/j.1399-0039.1995.tb02437.x
• EISSN: 1399-0039
• ISSN: 0001-2815
• Language: English
• Keywords: Alleles; Africa, Eastern; Gene Frequency; HLA-B Antigens; Molecular Sequence Data; HLA-C Antigens; DNA; Genes, MHC Class I; Base Sequence; African Continental Ancestry Group; Tumor Cells, Cultured; Polymerase Chain Reaction; HLA-A Antigens; Humans