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The HLA-A,B,C genotype of the class I negative cell line Daudi reveals novel HLA-A and -B alleles.

Abstract:

Daudi, a lymphoblastoid B cell line derived from an African Burkitt lymphoma does not express HLA-A,B,C antigens at the cell surface. Although HLA-A,B,C heavy chains are made normally they do not assemble into functional molecules because beta 2-microglobulin is absent. Previous serological analysis of somatic cell hybrids indicated that the HLA haplotypes of Daudi encoded HLA-A1, A10(A26), B17, and B16(38) antigens. Here we describe the application of molecular methods: ARMS-PCR, cDNA clonin...

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Publication status:
Published

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Journal:
Tissue antigens More from this journal
Volume:
45
Issue:
3
Pages:
177-187
Publication date:
1995-03-01
DOI:
EISSN:
1399-0039
ISSN:
0001-2815
Language:
English
Keywords:
Pubs id:
pubs:487583
UUID:
uuid:f7d66382-f4f2-45ec-b29e-75d3a966d25b
Local pid:
pubs:487583
Source identifiers:
487583
Deposit date:
2014-10-29

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